SPRING - May 2000 (and Fall 1999) NEWSLETTER


43rd Annual May Conference Review

by Jeanette Killius

The 43rd Annual May Conference took place May 19, 1999 at John Carroll University in University Heights. This is the first time MSNO has joined the Cleveland Section of the Society for Applied Spectroscopy and the Cleveland Section of the American Chemical Society Analytical Topics Group.

This daylong meeting began with opening remarks from Ron Baraona, president of SAS, and Mark Smith, president of MSNO. Concurrent sessions followed on Vibrational Techniques, NMR and UV-Vis, and Light Scattering Techniques.

After lunch, the keynote speaker, sponsored by a grant-in-aid from MSA, was Douglas Dorset from the Electron Diffraction Department at Hauptman-Woodward Medical Research Institute in Buffalo, NY. Dr. Dorset discussed "Crystallography with Electrons: Conflict and Rebirth". Dr. Dorset outlined the historical background of the development of electron diffraction as a science, from the 1930’s to present day, especially contrasting the efforts by Russian and American scientists in this process. When using crystallography, you must take care with experimental design and preparation so you are left with something workable. Specimen damage is a valid concern and he suggested techniques for combating this problem.

After Dr. Dorset’s presentation, the afternoon sessions were again split in two groups. Mark Smith chaired the Microscopy sessions. The first speaker was Ray Teller from BP who discussed a "user friendly" FE source SEM by LEO. This Windows 95 driven microscope has a beam very close to the specimen, a CCD camera and a Shottky detector so you can collect high resolution images with low voltage. The second presenter was MSNO member Judy Drazba. She gave a very interesting overview about confocal microscopy and different images she has taken in her work with the Cleveland Clinic Foundation. Morgan Sibbard of Goodyear described the "Multidisciplinary Character of Elastomer Blends". Morgan used several techniques to characterize different polymer blends - SPM, multi-angle laser light scattering and thermal flow fractionation among them.

MSNO member Bill Butler talked about an impression technique for plants to study them in the SEM. He used impression media to make a replica of the leaf and poured Spurr resin over it. After curing it overnight, he mounted it on a stub and gold coated it. The SEM results were very accurate. Finally, MSNO member Bill Landis presented fascinating work involving the creation of new phalanges by tissue engineering. He and his co-workers created a 3-D template of a finger bone with dissolvable suture and seeded it with osteoblasts. He also grew cartilage cells on sheets of dissolvable suture to stitch onto the ends of this model to create joint surfaces. The whole thing was sewn under the skin of a mouse to provide a blood supply for its growth. The resulting tiny bone was very life-like and promises a new venue for joint replacements. Ken Street of NASA’s Glenn Research Center discussed the advantages of FT-IR microscopy, especially when using a diamond anvil cell to flatten the sample. Among examples cited was work done with the Cleveland Museum of Art-using these tools helped identify the make-up of waxes and varnishes from an Egyptian temple.

The spring evening concluded with a reception and dinner at Notre Dame College. The after dinner speaker was Ed Pershey from the Western Reserve Historical Society. He gave a delightful look back at the rise and fall of the Cleveland streetcar. While the old streetcars are now gone, evidence of their presence still remains in the tracks of the city’s Rapid Transit system.

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Jeanette Killius Heads Tech Forum

by Jeanette Killius

At the recent Microscopy & Microanalysis 99 meeting in Portland, the reins of the Technologists’ Forum were officially handed from Bev Maleeff to MSNO’s Jeanette Killius. Jeanette has been Vice-Chair for the last three years of this special interest group within MSA. As chair, Jeanette’s principle responsibility will be to arrange the Tech Forum program at the annual Microscopy and Microanalysis meeting.

Membership to the Technologists’ Forum is open to any MSA member at no extra cost. The TF strives to bring programming to the national meeting aimed at improving technological understanding of current research. Additionally the TF has a booth on the show floor with meeting announcements, Hints ‘n Tips, and other helpful handouts. It is a place to chat with technologists and problem-solve. The TF puts out 2 newsletters annually to keep members aware of upcoming meetings, deadlines, informational articles and summaries of symposia. The TF sponsors a Web site that can be reached via a link within the MSA Web site (www.microscopy.com) under the "Committees and General Info" button.

There are several awards promoted by the Tech Forum. The Outstanding Technologist Award is given annually to both materials and biological scientists who have demonstrated exemplary careers in technology. The Professional Technical Staff Award is open to technologists in biological and materials research who submit an abstract to present at the M&M meeting. These abstracts are reviewed and winners selected who are reimbursed for registration and up to $600 for travel and housing expenses.

If you would like more information about the Technologists’ Forum or to become a member, please contact Jeanette Killius at jkillius@neoucom.edu or phone 330-325-6311.


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Student Receives Scholarship

by Jacques Gilloteaux, DSc.

The Microscopy Society of America has given a scholarship grant of $2500 for the training of one of my student, David Arnold, on a project involving scanning electron microscopy. The project has started last year and this support for our project is important, because David's contribution is to

accomplish a part of a study involving several microscopic techniques, i.e. phase, confocal, fluorescence, histochemistry and cytochemistry, scanning and transmission electron microscopy. The project itself involves the use of a new combined vitamin treatment against cancer cells. Vitamin C (VC), vitamin K3 (VK3) and a VC:VK3 combination were used to study the cellular and subcellular damages produced by these vitamins on cancer cells from human bladder, prostate and other cell lines. The study also includes ovarian carcinoma

cells. This scholarship allowed him to participate in this ongoing research, with Dr. J. Jamison and Dr. J.L. Summers (Summa Health Foundation, Akron OH), wherein we have uncovered a new kind of cell death for cancer cells, not apoptotic and not necrotic, that we named autoschizis. We would like to recognize the Microscopy Society for its efforts in promoting training and encouragement for undergraduate students to perform research with microscopic techniques. See photo page-David Arnold receiving his scholarship check from Dr. Gilloteaux. For more information, contact:

J. Gilloteaux DSc
Professor of Anatomy and Cell Biology
LECOM Erie PA.


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Newsletter Editor needed!

By Kathleen Doane, Ph.D.

Since our illustrious newsletter editor, Jeanette Killius, resigned from her long-held post to move on to bigger and better things (including running the Tech Forum for MSA and getting out their newsletter; see above article), MSNO has been searching for a newsletter editor. You would get to come to MSNO Board meetings, and the Board is considering a stipend for this position (no guarantees)! Any interested party please contact Kathleen J. Doane, Ph.D., President-elect of MSNO (330-325-6304; kd@neoucom.edu ).


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M&M 1999 Portland-Meeting Summary

By Jeanette Killius

The site of the 1999 joint meeting of the Microscopy Society of America and the Microbeam Analysis Society was Portland, Oregon - the City of Roses. The weather during that week in early August was a cool break from the blistering heat of the Midwest. The Opening Reception, held outdoors at the Oregon Zoo on the outskirts of the city, was a marvelous success due in no small part to the sunshine and pleasant temperatures in the 70's.

Sessions began in earnest Monday morning. The distinction of talk number 001 went to MSNO Trustee Bill Landis of NEOU College of Medicine. His invited presentation, "Development of Cartilage and Bone During Formation of Phalanges by Tissue Engineering", described the creation of a finger bone using molded dissolvable suture seeded with osteoblasts and chondrocytes and grown under the skin of a nude mouse to provide nutrition. In a talk later Monday morning, Meredith Bond and M. Ruehr discussed using fluorescence resonance energy transfer to make real-time measurements of protein kinase-dependent binding events in cardiac myocytes, which play a role in sympathetic regulation of heart muscle contraction.

MSA President David Joy chaired Monday afternoon's Presidential Happenings. He began by presenting several awards. The Distinguished Scientist Award in the Physical Sciences went to Takeo Ichinokawa. The Distinguished Scientist Award in the Biological Sciences was given to Sir Aaron Klug. The Burton Medal was presented to Z.L. Wang. Charles E. Lyman won the Morton D. Maser Distinguished Service Award. The Outstanding Technologist Awards went to John C. Wheatley (Physical Science) and John M. Basgen (Biological Science). Bev Maleeff, Technologists' Forum Chair, gave the Professional Technical Staff Awards to Wen-An Chiou, Michael Larsen, and Steven J. Schmitt. Following the awards presentations, renowned microscopist/artist David Scharf presented a slide show of his creative SEM images.

Tuesday was filled with papers and posters covering, among other things, confocal microscopy advances, ESEM, cryotechniques, and digital imaging. MSNO President Michael Mallamaci gave a platform presentation entitled "Interfaces in Glass-containing Ceramics". MAS continued their 2-day symposia celebrating 50 years of electron probe microanalysis dedicated to Professor R. Castaing and the Professor Howie symposia on pioneering EM. At the end of the day, MAS President John Friel chaired the MAS awards ceremony capped by Joseph Goldstein's talk on "SEM and Electron Probe Microanalysis of Extraterrestrial Material".

Also on Tuesday afternoon, new OTA winners John Basgen and John Wheatley summarized their research careers as part of the Tech Forum Special Topics session. John Basgen's talk, "25 Years in a Stereology Lab: a Point by Point History" gave a realistic view of stereology and measurement with TEM of kidney glomeruli. His mantra, "Do more, less well" means statistically it makes more sense to add more animals to the initial study group and count few points rather than relentlessly counting every single point in a very small pool of samples. Also, even with advances in computerization, it is just as quick and accurate to manually point-count areas with a grid. John Wheatley covered his 29 years in materials research by discussing "Factors Which Influence Success". These are: good financial support, students to teach, and an excellent machine shop. Funding provides new instruments, students do research and submit papers, and the machine shop helps rebuild aging equipment at a fraction of the cost. A measure of this success is the lab's continued ability to obtain funding.

The Technologists’ Forum Symposium "Technology from the Pacific Northwest" began Wednesday morning. The session was co-chaired by TF Chair Bev Maleeff and TF Vice-Chair (and MSNO member) Jeanette Killius. Bonnie Yates of the National Fish and Wildlife Forensics Lab in Ashland, Oregon, spoke on "Secondary Guard Hairs in Animal Hair Identification". She provided a fascinating look into the world of the wildlife forensics expert – from verifying the origins of fish caviar to matching parrot DNA to comparing animal hairs to authenticate pelts and clothing made from them. Bruce Wong of Wong’s Forensic and Metallurgical Engineers, Inc. discussed applications in metallurgical failure analysis. Broken screws, pitting of food container surfaces, collapse of ladder supports, underground pipe corrosion and bad microswitches are some of the things he has examined to determine each cause of failure using mostly SEM. Dr. Anda Cornea from the Oregon Primate Research Center presented an introduction to confocal microscopy. She described the function and uses of confocal microscopy, including tagging with green fluorescent protein to track live cells, multiphoton imaging, photobleaching and fluorescence resonance energy transfer. Terry McAdam of the Washington State Patrol showed how SEM/EDX examination of evidence found at the scene of a crime led to a conviction of one victim’s son-in-law for double murder. The Tech Forum Symposium concluded Thursday with Dr. Walt Metcalfe of Molecular Probes who discussed advantages and disadvantages of working with probes and dyes in his talk "Detecting and Optimizing Fluorescence Signals". Each stain (ex. DiI, DAPI, Alexa Fluor, etc.) presented challenges for optimization of the proper dye amount in a system and calibration of the microscope for efficient signal detection.

The highlight of the Exhibit Hall was the new MSA megabooth. Bev Maleeff, TF Chair, coordinated its production. This is the first time all the different subcommittees of MSA (Education, Tech Forum, Project MICRO, Job Placement, Certification and Membership) were featured in the same island. Each committee received a new display and was placed at a corner with Education occupying the center. The heart of the booth was a 12’ lit tower with the "MSA" logo atop – visible from anywhere on the show floor. It received very positive reaction from attendees! Look for it next year in Philadelphia.

M&M99 in Portland was the most successful meeting to date with over 1850 attendees. The next MSA meeting will be August 13-17, 2000 in Philadelphia. See the preview article elsewhere in this newsletter.


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1999 National Election Results for MAS and MSA

By Valerie Woodward

 

The Microbeam Analysis Society elected the following individuals in the December 1999 elections:

Richard W. Linton, University of North Carolina, Chapel Hill – president-elect

Edgar S. Etz, NIST – Secretary

Richard Leapman, NIH – Director, 2000-2002

Valerie Woodward, BFGoodrich (MSNO) – Director, 2000-2002

The Winter Council meeting was held in Savannah, GA, January 21-22, 2000. Major topics discussed were M&M 2000 in Philadelphia, IUMAS in Kona, Hawaii, and MAS topical conference support. Of note, Ian Anderson, MAS Director 1999-2001, will be our speaker (supported by the MAS Tour Speakers Program) at the Spring Symposium on May 16 at the Winery at Wolf Creek, Norton, OH.

The Microscopy Society of America elected the following individuals in the December 1999 elections:

Ron Anderson, IBM retired – President-elect

Janet Woodward, Buckman Laboratories – Secretary, 2000-2002

Sara Miller, Duke Medical Center – Director, 2000-2002

Tom Kelly – Director, 2000-2002

Check out the national society’s websites for more details on the officers, meetings and affiliated societies:

MSA – www.microscopy.com; and MAS – www.microanalysis.org


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Fall 1999 Symposium at Akron Rubber Development Laboratory

By Valerie Woodward

We would like to thank the Akron Rubber Development Lab for hosting and co-sponsoring the Fall Symposium, and Vince Giangarlo of Giangarlo Scientific and Andre Domino of B and B Microscopes for sponsoring the social hour. The meeting was very well attended, and presented an excellent cross section of microscopy and microanalytical techniques for biologists and physical scientists. The biological speakers were Mike Marko of the Wadsworth Center, NIH Albany, and Dr. Ron Midura, of the Cleveland Clinic Foundation. Their respective talks were Recent Advances in Electron Tomography (cryo-TEM) and Fluorescent Detection and Quantification of Calcium Minerals in Biological Specimens. The physical science speakers were Dr. Charles Herd, Columbian Chemicals Company, and Dr. Sergei Magonov, Digital Instruments, Inc., who spoke about Carbon Black Morphology as Determined Using TEM with Automated Image Analysis and Visualization of Polymer Morphology and Nanostructures with AFM, respectively. The physical science speakers were supported by ARDL; the biological speakers were supported by MSNO.

This meeting was also the inaugural appearance of the new MSNO coffee mugs! Thanks to Jeanette Killius for her hard work in coming up with the design, contacting the vendors and making the mugs happen!


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MSNO Member Joe Polak Obituary

By Valerie Woodward

On October 29, 1999, long-time MSNO member Joe Polak passed away after a short illness. Many MSNO members will remember Joe from his days as EM lab manger at Case Western Reserve University, and most fondly as the organizer of MSNO golf outings, especially the M&M ’97 golf outing when Cleveland hosted the national meeting. His wife, Sharon, daughters Kelsey, Rachel and Megan, parents Eleanor and Charles, and brothers Michael and David survive Joe. Joe’s smiling face and outgoing personality will be sorely missed by his fellow members and friends.

As a memorial to Joe, the society has donated $100.00 to the Polak Children’s College Fund. If anyone would care to make an individual contribution, they can be directed to: Polak Children’s College Fund, c/o Larry Kline Pension Consulting Services, 1315 Corporate Dr., Hudson, OH, 44236.


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Spring 2000 meeting at Wolf Creek Winery

By Valerie Woodward

The Spring 2000 meeting will be Tuesday, May 16, 2000 at the Winery at Wolf Creek in Norton, OH. Our speaker will be Ian Anderson of ORNL, presenting Spectrum Imaging. There will also be a winery tour and tasting in conjunction with the technical presentation.

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Microscopy Technology Center Donation

By Kathleen J. Doane, Ph.D.

MSNO has contributed to a worthy building project at San Joaquin Delta College in Stockton, CA. This college’s Microscopy Technology Program, a unique program which provides well-trained students for numerous high-tech jobs in the California area, is in the process of building a $7.5 million Microscopy Technology Center.

Although funding from the state provided a large percentage of the cost, to raise the additional $3 million necessary the college was selling commemorative building tiles. MSNO contributed $250 to purchase a tile with our name inscribed. Judy Murphy, of the Microscopy Technology Center, sent a letter thanking MSNO for our generous contribution, which will facilitate their efforts to train students in high-tech microscopy technologies. She also invited MSNO members to visit the Microscopy Technology Center if they are ever in the Stockton CA area.


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MSNO Bylaws Changes

By Kathleen J. Doane, Ph.D.

Several changes to the MSNO bylaws were proposed to the membership and were approved at the March 7 "Mardi Gras" meeting at Goodrich. One bylaw, which originally mandated 4 meetings per year, was changed to requiring 3 meetings per year, reflective of the way the Society has operated in the past few years. Another change involved including all members in various voting procedures and Board positions, regardless of membership type. The original wording of the bylaws stated that only "regular" members could participate in certain votes or hold a Board membership. One important change involved election of Board members. Elections will no longer be held at the Spring MSNO meeting. The new bylaw calls for elections by a mail ballot, with candidates who receive a majority vote being elected. MSNO members should have received these ballots and voted for Board members for the upcoming fiscal year. Results will be announced at the Spring May 16th meeting at Wolf Creek Winery. This change helps to give the MSNO membership a greater say in the functioning of our organization. Other changes include: making the newsletter editor a voting board member (note the advertisement for this position elsewhere in this newsletter); allowing the Society to affiliate with other Societies with a vote by the Board, and streamlining the procedures for adding amendments to bylaws. These bylaws changes will help keep our Society one of the most vital of all affiliate societies of MSA.


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Student Members Needed

by Jeanette Killius

Attention students and advisors: MSNO is undertaking a membership drive to increase the number of students in our society. During the coming year, MSNO would like to encourage student participation by hosting a platform/poster competition with cash prizes for the best presentations. Membership in our local society allows students to interact with other microscopists across NE Ohio and give them the opportunity to hear local and national speakers. Talks are always free, dinner is optional. This valuable learning tool is available to students for only $5/year membership - a real bargain! Please copy the membership form in this newsletter and hand it to every student in your lab. Help assure MSNO's future!


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March 2000 Meeting Review

By Jeanette Killius

BF Goodrich in Brecksville hosted the Member Speaker Night on March 7th. Prior to the talks guests enjoyed a wine and cheese reception sponsored by vendor member Electron Microscopy Sciences.

The first presentation featured a talk by University of Akron Professor Mark Foster. Dr. Foster used scanned probe microscopy, nanoprobe and microindentation to show surface changes in pressure-sensitive adhesives with regards to composition, over time, and after application of heat. The adhesive is composed of a tackifier plus a rubbery matrix. By changing the percentage of tackifier, you change the composite structure of the adhesive and thereby its sticky properties. These adhesives slowly polymerize with time or after heat causing them to coarsen and stiffen and thus lose their tackiness.

A short business meeting was held. President Mike Mallamaci discussed the changes in MSNO's bylaws (outlined more fully elsewhere in this newsletter). The changes were voted on by the membership present and passed.

Taking advantage of the season, dinner featured a delightful Mardi Gras buffet complete with decorated tables and jazz music in the background. Blackened chicken, shrimp jambalaya and freshly-made Bananas Foster highlighted the meal.

After dinner, Dr. Jacques Gilloteaux of the Lake Erie College of Osteopathic Medicine presented the second talk of the evening. Dr. Gilloteaux and his collaborators used a variety of microscopy techniques, including fluorescence, SEM and TEM, to study the cytotoxic effects of Vitamins C and K on cancer cells in tissue culture. Administered individually, these vitamins cause the cells to flatten by collapsing the actin cytoskeleton. But together, the cells extrude their cytoplasm and organelles leaving a shrunken inactive nucleus. This new kind of cell death is termed autoskizis. In vivo, this treatment caused tumors in nude mice to shrink away without harming healthy tissue, giving hope for a magic bullet cancer cure.

We wish to thank Electron Microscopy Sciences for sponsoring the reception and BF Goodrich for hosting the meeting. MSNO's next meeting is on Tuesday, May 16th at Wolf Creek Winery. The featured speaker is Ian Anderson.


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M&M is Rapidly Approaching

by Jeanette Killius

Philadelphia, the Place that Loves You Back, is host to M&M 2000. This joint conference of MSA, MAS, and MSC takes place August 13-17 at the Philadelphia Convention Center. The program has been

set and will feature platform and poster presentations in areas such as: STEM, EELS, FIB, SPM, ESEM, LVSEM, ceramics, polymers, labeling, vascular casting, biomaterials, microorganisms and cancer biology. The popular "Ask the Experts" sessions for biological and physical scientists will be back. This year's Tech Forum symposium covers the how-to's of Cryo microscopy. The meeting will also feature the largest assortment of microscopy equipment yet in the Exhibit Hall. See the latest instruments each company has to offer.

For those who arrive early, several short courses are available on Sunday. Basic TEM, Basic SEM, Introduction to X-ray EDS, Practical Digital Imaging and the Digital Darkroom will be offered. In addition, a special 2-day congress (Saturday and Sunday) on SPM of Soft Material will be held. Separate registrations fees are needed for all these courses.

If you would like to relax a bit before or during the conference, take advantage of the social events put together at special rates for meeting-goers. Enjoy a round of golf with friends Saturday morning at the 13th annual golf outing to be played at Wyncote Golf Club. The $80 fee includes transportation, greens fees, cart and a box lunch. Saturday afternoon features a fun jaunt to Atlantic City's casinos. Roundtrip bus fare is only $37. The Sunday night reception will showcase Philly's ethnic diversity with food and entertainment at the Philadelphia Convention Center. Full registration includes a ticket to this festive event. Extras are available for $50. Come on out the ballpark Tuesday night! For $32.50, you can root for the Phillies as they take on the Arizona Diamondbacks. Your ticket includes a box seat, transportation, hot dogs and soft drinks. Contact Bev Maleeff at Beverly_E_Maleeff@sbphrd.com to reserve your seat. The annual dinner cruise is Wednesday night. Tour the Delaware River aboard the Spirit of Philadelphia while enjoying a dinner buffet and dance band. Tickets are $55. All events require early reservations.

To obtain information about the conference, to register for M&M 2000 or for housing info, check the web site at www.microscopy.com/MSAMeetings/MMMeeting.html. See you all in Philly!!!


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UPCOMING MEETINGS AND WORKSHOPS

Lehigh Microscopy Courses – 2000

For more information, contact Sharon Coe, Department of Materials Science and Engineering, 5 East Packer Ave., Bethlehem, PA 18015-3195; (610) 758-5133; FAX (610)758-4244; sharon.coe@lehigh.edu; http://www.lehigh,edu/~inmatsci/Microcourses.html.

Introduction to SEM and EDS for the New SEM Operator, June 11, 2000

Scanning Electron Microscopy and X-Ray Microanalysis, June 12-16, 2000

Advanced Scanning Electron Microscopy for Experienced Users, June 19-23, 2000

Quantitative X-Ray Microanalysis of Bulk Specimens and Thin Films on Substrates, June 19-22, 2000

Analytical Electron Microscopy Analysis Methods for TEM Specimens, June 19-22, 2000

Microdiffraction: Electron and X-Ray Techniques, June 19-23, 2000

Atomic Force Microscopy and Other Scanned Probe Microscopies, June 20-23, 2000

Cryo SEM: Low-Temperature Microscopy and Analysis, June 21-23, 2000

Advances in Molecular Labels, Signaling & Detection: Enhancing Sensitivity, Accuracy and Speed, Washington, D.C., June 12-13, 2000. For more information, contact the Cambridge Healthtech Institute, 1037 Chestnut Street, Newton Upper Falls, MA 02464; (617) 630-1300 OR (888) 999-6288; FAX (617) 630-1325.

Explomet 2000, Albuquerque, NM, June 19-23, 2000; Conference on shock-wave and high-strain-rate phenomena effects on materials. For more information, contact Dr. Karl P. Staudhammer, Los Alamos National Laboratory, PO Box 1663, MS G770 Los Alamos, NM 87545; (505)667-9333; FAX (505) 665-3748; staudhammer @lanl.gov

5th Annual 11-Day Short Course on 3D Microscopy of Living Cells and Post Course on 3D Image Processing, Vancouver, British Columbia, Canada, June 19-29, 2000. For more information, contact Dr. Elaine Humphrey, Biosciences EM Facility, Biosciences Building, University of British Columbia, 6270 University Blvd., Vancouver, BC V6T-IZ4, Canada; www: http://www.cs.ubs.ca/spider/ladic/course/forms/register.txt

AUSTCERAM 2000 (the Australasian Ceramic Society), Sydney, Australia, June 25-28, 2000. For more information, contact The Secretariat, AUSTCERAM 2000 c/o Ariela Travel, 334 Elizabeth St, Surry Hills, NSW 2010, Australia; 61-2-9211-0061 (PH and FAX); Aust.Ceram2K@uts.edu.au; WWW: http://www.science.uts.edu.au.Austceram2K/

Inter/Micro – 2000, Chicago, IL, June 26-29, 2000. Conference on all aspects of microscopy. For more information, contact Nancy Daerr, McCrone Research Institute, 2820 South Michigan Avenue, Chicago, IL 60616; (312) 842-7100; FAX (312) 842-1078; ndaerr@mcri.or.

7th Asia-Pacific Conference on Electron Microscopy, Singapore. Malaysia, June 26-30, 2000. For more information, contact the 7th APEM Organizing Committee, c/o Electron Microscopy Unit, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260; 65 874 3216 or 3282; FAX 65 776 4971; micngml@nus.edu.sg or medlab2@nus.edu.sg; WWW: http:www.med.nus.edu.sg/micsoc/7apem

International Kunming Symposium on Microscopy, Kunming, P.R. China, July 2-5, 2000. For more information, contact the IKSM Office, Institute of Physics, #37, Chinese Academy of Sciences, P.O. Box 603, Beijing 100080, P.R. China; 86 10 8264 9170; FAX 86 10 8264 9531; IKSM@aphy.iphy.ac.cn

Ultrastructural Pathology X, Florence, Italy, July 2-7, 2000. For more information, contact Professor Marco Santucci, Universita degli Studi di Firenze, Instituo di Anatomia e Istologia Patologica, V.le G.B. Morgagni 85-50134, Firenze, Italy; 055 432`44; FAX 055-4379868; Santucci@CESIT1.UNIFI.IT.

IUMAS 2000, Kona, Hawaii, July 8-15, 2000; Meeting of the International Union of Microbeam Analysis Societies; For more information contact David B. Williams; (215) 758-4224; FAX (215) 758-4244; DBW@lehigh.edu

12th European Congress on Electron Microscopy, Brno, Czech Republic, July 9-14, 2000; For more information, contact Petr Schauer; (420 5) 41514313; FAX (425 5) 41514404; petr@isibrno.cz OR csem@isibrno.cz; WWW: http://www.eurem2000.isibrno.cz/

Microscopy and Microanalysis 2000, August 13-17, 2000, Philadelphia, PA; For more information, contact M&M Meeting Management (708) 361-6045; Toll free (877) MSA-MAS-1; FAX (708) 361-6166; msa@tradeshownet.com. Preregistration discounts before 7/14 for members the meeting will cost $260; after 7/14 the cost rises to $325.

Joint Meeting of the Brazilian Society for Microscopy and Microanalysis, Brazilian Society for Cell Biology, and the Portuguese Societies for Electron Microscopy and Cell Biology and Anatomy, Goiania, Brazil, August 26-September 2, 2000. For more information: com_morf_2000@icb1.ufg.br OR sbmm.ceme@epm.br OR microcel@biomed.icb2.usp,br.

6th International Conference on Near-Field Optics and Related Techniques, University of Twent, Enschede, The Netherlands, August 27-31, 2000. For more information, contact NFO-6 Conference Secretariat CAT, J.A. Spierenburg, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands; 31 53489 444; FAX 31 53489 4442; j.a.spierenburg@basics.utwent.nl; WWW: http://www.tn.utwente.nl.nfo-6

11th International Congress of the Federation of Societies for Histochemistry and Cytochemistry, University of York, UK, September 3-8, 2000. For more information, contact Rebecca Morden at the Royal Microscopical Society, 37/38 St. Clements, Oxford OX4 IAJ, UK; 44(0) 1865 248768; FAX 44(0) 1865 791237; info@rms.org.uk; WWW: http://www.med.ic.ac.uk\external\ichc_2000\

Applied Optics and Optoelectronics Conference, Loughborough, UK, September 17-21, 2000. For more information, contact the Institute of Physics, 76 Portland Place, London WIN 3DH, UK; 44 171 470 4800; FAX 44 171 470 4848; conferences@iop.org

TMS (The Minerals, Metals & Materials Society) Fall Meeting, St. Louis, Missouri, October 8-12, 2000. For more information, contact the TBS Customer Service Center, 184 Thorn Hill Road, Warrendale, PA 15096-7528; 800-759-4867 or (724) 776-9000, ext. 270; FAX (724)776-3770; csc@tms.org; WWW: http://www.tms.org/Meetings/Fall99/Fall99.html


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