MSNO Summer School 2015
Theme: Microscopy of Soft-Matter Materials
Time and place: July 17th @ Liquid Crystal Institute, Kent State University
Goal: To promote the basic knowledge of light microscopy, basic electron microscopy (EM) and cryo-EM techniques for soft-matter materials (biological materials, polymers, liquid crystals, etc.)
Instructors: Dr. Min Gao (Kent State University), Dr. Adam Smith (University of Akron), Dr. Midori Hitomi (Cleveland Clinic)
Topics:
1. Optical microscopy: optical path and camera basics (Smith)
2. Basics of electron microscopy – a quick overview (Gao)
3. Fluorescence imaging: Theory and advanced applications (Smith)
Registration: All the attendees need to register online before the meeting. The online registration will be closed at 11:59pm July 15. If you have any trouble and questions with the online registration, please contact us. The registration is free to MSNO members.
Contacts: Please contact Dr. Lu Zou ([email protected]) if you have general questions. Please direct all your technical questions to Min Gao ([email protected], 330-672-7999).
Tentative Schedule:
8:30-9:00 Registration
9:00-9:45am Optical microscopy: optical path and camera basics (Adam Smith, University of Akron)
10:00-10:45 Basics of electron microscopy – a quick overview (Min Gao, Kent State University)
11:00-11:45 Fluorescence imaging: Theory and advanced applications (Smith)
12:00-1:15pm Lunch
1:15-1:45 Biological Electron Microscopy: Things you should know to obtain best images (Midori Hitomi, Cleveland Clinic)
2:00-2:45 Cryo-EM of soft-matter materials (Gao)
3:00-3:45 Cryo-EM specimen preparation for soft-matter materials (Gao)
4:00-5:00pm Demos and discussion
Drinks, refreshment, and lunch (pizza) will be provided. If you have any special needs, please let us know.
Time and place: July 17th @ Liquid Crystal Institute, Kent State University
Goal: To promote the basic knowledge of light microscopy, basic electron microscopy (EM) and cryo-EM techniques for soft-matter materials (biological materials, polymers, liquid crystals, etc.)
Instructors: Dr. Min Gao (Kent State University), Dr. Adam Smith (University of Akron), Dr. Midori Hitomi (Cleveland Clinic)
Topics:
1. Optical microscopy: optical path and camera basics (Smith)
2. Basics of electron microscopy – a quick overview (Gao)
3. Fluorescence imaging: Theory and advanced applications (Smith)
- What is fluorescence, and why is it commonly used for biological samples?
- Single particle/molecule tracking
- Correlation methods
- Chemical fixation and ultramicrotomy
- What EM facility users can do and/or should not do to obtain high quality images
- To understand the importance of 1) sample preparation, 2) interpretation of data, and 3) communication between the user and the EM facility staff.
- Basics and application examples of plunge-freezing, high pressure freezing, cryo-ultramicrotomy, and freeze fracture.
- Low-dose cryo-EM.
- Touching upon: freeze substitution, correlated light and electron microscopy (CLEM), EM-tomography, etc.
Registration: All the attendees need to register online before the meeting. The online registration will be closed at 11:59pm July 15. If you have any trouble and questions with the online registration, please contact us. The registration is free to MSNO members.
Contacts: Please contact Dr. Lu Zou ([email protected]) if you have general questions. Please direct all your technical questions to Min Gao ([email protected], 330-672-7999).
Tentative Schedule:
8:30-9:00 Registration
9:00-9:45am Optical microscopy: optical path and camera basics (Adam Smith, University of Akron)
10:00-10:45 Basics of electron microscopy – a quick overview (Min Gao, Kent State University)
11:00-11:45 Fluorescence imaging: Theory and advanced applications (Smith)
12:00-1:15pm Lunch
1:15-1:45 Biological Electron Microscopy: Things you should know to obtain best images (Midori Hitomi, Cleveland Clinic)
2:00-2:45 Cryo-EM of soft-matter materials (Gao)
3:00-3:45 Cryo-EM specimen preparation for soft-matter materials (Gao)
4:00-5:00pm Demos and discussion
Drinks, refreshment, and lunch (pizza) will be provided. If you have any special needs, please let us know.
